Award details

Isothermal titration calorimetry: novel and established applicatons in redox enzymology

Reference	REI20488
Principal Investigator / Supervisor	Professor Nigel Scrutton
Co-Investigators / Co-Supervisors	Dr Aldo Gutierrez, Professor David Leys, Professor Peter Moody, Professor Andrew Munro, Professor Emma Raven, Professor Gordon Roberts
Institution	University of Leicester
Department	Biochemistry
Funding type	Research
Value (£)	29,252
Status	Completed
Type	Research Grant
Start date	01/10/2003
End date	31/03/2004
Duration	6 months

Abstract

This application requests funding to establish a facility for anaeronic isothermal titration calorimetry within the REAL (Redox Enzymology at Leicester) consortium at the University of Leicester. The equipment will support a number of mature research projects funded by BBSRC and the other grant awarding agencies focussed on the mechanisms of redox enzyme catalysis, and will involve studies of protein-ligand, protein-protein interaction, and also kinetic studies. The instrument will be housed in an anaerobic glove box for studies of redox enzymes in an oxygen-free environment. This will enable studies with oxygen labile substrates and interactions in different redox states of the protein. These interaction studies are required to address a new mechanistic proposals arising from structural and kinetic studies performed at Leicester both on redox enzymes, and on other important enzyme and signalling proteins. The instrument will be maintained in a new kinetics laboratory with existing transient kinetic instrumentation in a new 22M Biomedical Sciences Research Building (completion June 2004) in which the REAL group members will be accommodated.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2003 (RE2) [2003]
Funding Scheme	X – not Funded via a specific Funding Scheme

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