Award details

Conformational analysis of biomolecules by CD spectroscopy: structural and dynamic studies

Reference	JEI09276
Principal Investigator / Supervisor	Professor Nigel Scrutton
Co-Investigators / Co-Supervisors	Professor Peter Andrew, Dr Shaun Heaphy, Dr Robert Liddington, Professor Anthony Maxwell, Professor Gordon Roberts
Institution	University of Leicester
Department	Biochemistry
Funding type	Research
Value (£)	42,504
Status	Completed
Type	Research Grant
Start date	15/04/1998
End date	15/10/1998
Duration	6 months

Abstract

This proposal aims to establish state-of-the-art circular dichroism spectroscopy instrumentation within the life sciences community at Leicester. Many groups are committed to the study of protein structure and function, and this area of research is perceived as one of the major strengths of Leicester's research activity. Circular dichroism spectroscopy is central to the study of macromolecular structure, especially for assessing the folded state of heterologous proteins produced by recombinant means. CD spectroscopy is not available at Leicester and research groups are presently forced to use facilities at other universities e.g. Newcastle, Cambridge, Mill Hill and Stirling. For routine work and for studies of labile biological molecules, this situation is inadequate and more sophisticated work (e.g. dynamic studies) is not possible without 'in-house' apparatus. One of the advantages of the Jasco machine is that it can be fitted with stopped flow attachments enabling transient kinetic measurements to be made in addition to equilibrium CD. A major use of the equipment will be in assessing the folded state of recombinant (often labile) proteins prior to structural studies by x-ray crystallography. NMR spectroscopy and hydrodynamic methods, and also for kinetic and stability studies of protein-cofactor assembly, protein-protein and protein-nucleic acid interactions.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Research Equipment Initiative 1997 (JE2) [1997]
Funding Scheme	X – not Funded via a specific Funding Scheme

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