Award details

Use of frog oocyte and egg extracts to reprogramme nuclear function in mammalian cells

ReferenceG20291/2
Principal Investigator / Supervisor Professor Ian Wilmut
Co-Investigators /
Co-Supervisors
Professor Helen Sang
Institution University of Edinburgh
DepartmentReproductive and Developmental Sciences
Funding typeResearch
Value (£) 146,869
StatusCompleted
TypeResearch Grant
Start date 01/07/2005
End date 31/03/2007
Duration21 months

Abstract

There is a far greater than expected developmental plasticity in mammalian cells, including that shown after nuclear transfer. However, in present procedures only a small proportion of cells or nuclei are reprogrammed appropriately. In this collaborative application we propose to investigate the ability of oocyte and egg extracts to reprogramme mammalian nuclei before either cell culture or nuclear transfer. We will overcome the limitation imposed by the small quantity of material available from mammalian eggs by taking advantage of the ability of frog extracts to reprogramme gene expression in mammalian nuclei. Reprogramming will be assessed by observations of gene expression and of the ability of treated cells to support development after nuclear transfer. (Joint with grant number 20421).

Summary

unavailable
Committee Closed Committee - Genes & Developmental Biology (GDB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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