Award details

Use of frog oocyte and egg extracts to reprogramme nuclear function in mammalian cells

Reference	G20291
Principal Investigator / Supervisor	Professor Ian Wilmut
Co-Investigators / Co-Supervisors	Professor Helen Sang
Institution	The Roslin Institute
Department	Gene Expression and Development
Funding type	Research
Value (£)	249,524
Status	Completed
Type	Research Grant
Start date	01/01/2004
End date	30/06/2005
Duration	18 months

Abstract

There is a far greater than expected developmental plasticity in mammalian cells, including that shown after nuclear transfer. However, in present procedures only a small proportion of cells or nuclei are reprogrammed appropriately. In this collaborative application we propose to investigate the ability of oocyte and egg extracts to reprogramme mammalian nuclei before either cell culture or nuclear transfer. We will overcome the limitation imposed by the small quantity of material available from mammalian eggs by taking advantage of the ability of frog extracts to reprogramme gene expression in mammalian nuclei. Reprogramming will be assessed by observations of gene expression and of the ability of treated cells to support development after nuclear transfer. (Joint with grant number 20421).

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

G20291/2 Use of frog oocyte and egg extracts to reprogramme nuclear function in mammalian cells

G20421 Use of frog oocyte and egg extracts to reprogramme nuclear function in mammalian cells

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