Award details

Use of frog oocyte and egg extracts to reprogramme nuclear function in mammalian cells

Reference	G20421
Principal Investigator / Supervisor	Professor Julian Blow
Co-Investigators / Co-Supervisors
Institution	University of Dundee
Department	College of Life Sciences
Funding type	Research
Value (£)	177,000
Status	Completed
Type	Research Grant
Start date	15/03/2004
End date	14/03/2007
Duration	36 months

Abstract

There is a far greater than expected developmental plasticity in mammalian cells, including that shown after nuclear transfer. However, in present procedures only a small proportion of cells or nuclei are reprogrammed appropriately. In this collaborative application we propose to investigate the ability of oocyte and egg extracts to reprogramme mammalian nuclei before either cell culture or nuclear transfer. We will overcome the limitation imposed by the small quantity of material available from mammalian eggs by taking advantage of the ability of frog extracts to reprogramme gene expression in mammalian nuclei. Reprogramming will be assessed by observations of gene expression and of the ability of treated cells to support development after nuclear transfer. (Joint with grant number 20291).

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

G20291 Use of frog oocyte and egg extracts to reprogramme nuclear function in mammalian cells

G20291/2 Use of frog oocyte and egg extracts to reprogramme nuclear function in mammalian cells

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