Award details

Elucidating the mechanism of explosive degradation by PETN reductase

Reference	C13574
Principal Investigator / Supervisor	Professor Neil Bruce
Co-Investigators / Co-Supervisors	Professor Peter Moody, Professor Nigel Scrutton
Institution	University of York
Department	Biology
Funding type	Research
Value (£)	27,951
Status	Completed
Type	Research Grant
Start date	01/01/2001
End date	01/01/2004
Duration	36 months

Abstract

We have solved the structure of the explosive degrading enzyme PETN reductase at high resolution and also specific complexes with inhibitors and explosive molecules. We will now exploit this structural information by investigating the novel mechanisms of explosive degradation using a combination of site-directed mutagenesis, X-ray crystallography, transient kinetic and solution studies. We will probe the role of Trp-102, which forms a novel covalent link with nitroaromatic explosives, and several other residues implicated in catalysis. We will also address the chemical mechanism of triazine explosive degradation and investigate the multiple chemical mechanisms available from nitroaromatic degradation. (Joint with grant 91/C13573.)

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

C13573 Elucidating the mechanism of explosive degradation by PETN reductase

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