BBSRC Portfolio Analyser
Award details
Deconvoluting the electron transfer pathway in flavocytochrome P450 BM3
Reference
B16808
Principal Investigator / Supervisor
Professor Andrew Munro
Co-Investigators /
Co-Supervisors
Professor Nigel Scrutton
Institution
University of Leicester
Department
Chemistry
Funding type
Research
Value (£)
200,428
Status
Completed
Type
Research Grant
Start date
21/01/2002
End date
20/07/2005
Duration
42 months
Abstract
B. megaterium flavocytochrome P450 BM3 is a soluble, self-contained P450, where a mammalian-like cytochrome P450 reductase is fused to a fatty acid hydroxylase P450 in a single polypeptide. It is an excellent system by which mechanism and electron transfer (et) rates can be determined in a eukaryotic-like (class II) P450 system. Previous work has inadequately/incorrectly described the et processes, and an impossible pathway for P450 et was proposed. We will examine et pathway/kinetics, using sited-directed mutagenesis to determine roles of key amino acids, and stopped-flow absorption/fluorescence, T-jump and laser flash photolysis to determine rates for individual catalytic steps. Newly developed stopped-flow analysis systems will permit analysis of rates of up to 3000/s and allow us to deconvolute accurately mechanism and kinetics in this model P450 system.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search