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Three-dimensional structure of the vacuolar H+-ATPase

Reference	B14097
Principal Investigator / Supervisor	Professor John B C Findlay
Co-Investigators / Co-Supervisors	Dr Michael Harrison, Professor Steve Homans, Professor Simon Phillips, Professor John Trinick
Institution	University of Leeds
Department	Inst of Molecular & Cellular Biology
Funding type	Research
Value (£)	1,021,012
Status	Completed
Type	Research Grant
Start date	01/01/2001
End date	01/01/2006
Duration	60 months

Abstract

The vacuolar H+-ATPase is a multi-subunit proton pump which is essential to eukaryotic cells. Its dysfunction and regulation are key elements in some human conditions. In this study, it is proposed to build on previous experience with the complex and its component subunits, to solve to atomic levels, the three- dimensional structure of subunits, sub-complexes and the intact assembly. This will be achieved by a combination of X-ray crystallography, NMR spectroscopy, electron microscopy and protein chemistry using native and expressed material. Each discipline will be directed by established experts in their respective fields. The information generated will allow the mechanism of action of this complex molecular motor to be elucidated and will facilitate the development of novel agents to regulate its activity.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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