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A novel genome-wide evaluation of mutants in samonella enterica serovar Typhimurium: application to drug and vaccine development

ReferenceAPG19114
Principal Investigator / Supervisor Professor Alastair Hawkins
Co-Investigators /
Co-Supervisors		 Professor Duncan Maskell, Professor David Stammers
Institution Newcastle University
DepartmentInst for Cell and Molecular Biosciences
Funding typeResearch
Value (£) 288,340
StatusCompleted
TypeResearch Grant
Start date 01/09/2003
End date 30/09/2006
Duration37 months

Abstract

Objective 1: To generate mutant libraries using two novel genome- wide technologies (TMDH and ODM) developed by Arrow Therapeutics. Objective 2: To identify genes important for bacterial survival, and thereby possible candidates for anti-microbial drug discovery. Attenuated mutants will be evaluated as candidate lives vaccines. The use of S. typhimurium allows the evaluation of survival of mutants in a real infection in an animal host, and thus defines unequivocally the minimum gene set required for virulence and persistence during infection. Objective 3: To select genes identified by TMDH and ODM for recombinant expression and characterisation. This approach utilises a set of proprietary bioinformatics tools along with protein purification, crystallisation methodologies, and structural determination to evaluate rapidly proteins for the drug discovery process. (Joint with grants 19115 and 19116).

Summary

unavailable
Committee Closed Committee - Genes & Developmental Biology (GDB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative LINK: Applied Genomics (APG) [2000-2004]
Funding SchemeX – not Funded via a specific Funding Scheme
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