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Novel use of bioluminescent bacteria immobilised on surfaces for rapid toxicity testing of wastes for disposal to arable land
Reference
SYS04659
Principal Investigator / Supervisor
Professor Ken Killham
Co-Investigators /
Co-Supervisors
Institution
University of Aberdeen
Department
Plant and Soil Science
Funding type
Research
Value (£)
107,578
Status
Completed
Type
Research Grant
Start date
01/01/1996
End date
01/01/1999
Duration
36 months
Abstract
Bioluminescence-marked (chromosomal integration of lux genes) bacterial (Rhizobia and Pseudomonads) biosensors will be immobilised on to surfaces (paper, plastic, fabric and optical fibre) prior to freeze-drying. The bioluminescence response of resuscitated, immobilised cells to a range of toxins (heavy metals and organoxenobiotics) will then be luminometrically assessed to identify the optimum test surface. Digestion (acid and H2O2) and extraction (acetone/hexane) procedures will be developed to recover toxins from wastes (sewage, distillery and paper waste) to enable them to be ecotoxicity tested (using the new biosensor technology) to assess suitability for application to arable land.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Systems - ROPA (SYS) [1994]
Funding Scheme
X – not Funded via a specific Funding Scheme
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