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Structural biology and design of the Greek key fold
Reference
SBD07536
Principal Investigator / Supervisor
Professor Sheena Radford
Co-Investigators /
Co-Supervisors
Institution
University of Leeds
Department
Inst of Molecular & Cellular Biology
Funding type
Research
Value (£)
158,991
Status
Completed
Type
Research Grant
Start date
29/09/1997
End date
29/09/2000
Duration
36 months
Abstract
The aim is to elucidate the structural determinants of the Greek key fold, using pseudoazurin as a model protein. Specific questions to be addressed are the role of loops; the importance of secondary structural propensity; and the involvement of hydrophobic interactions and strand docking in folding. In addition, we will seek experimental verification of two different models proposed on theoretical grounds to explain the folding of the Greek key. More elaborate experiments will involve the expression of a small Greek key subdomain, predicted to fold independently, for further redesign experiments. The structure of the mutant proteins will be determined by NMR methods and their stabilities and folding properties will be analysed by a wide range of biophysical methods.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Structural biology and design applications (SBD) [1996]
Funding Scheme
X – not Funded via a specific Funding Scheme
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