Award details

Infrastructure for single-molecule biology in the Manchester interdisciplinary biocentre

Reference	REI20500
Principal Investigator / Supervisor	Professor Douglas Kell
Co-Investigators / Co-Supervisors	Professor Jeremy Paul Derrick, Professor Andrew Doig, Professor John McCarthy, Professor Jason Micklefield
Institution	The University of Manchester
Department	Chemistry
Funding type	Research
Value (£)	113,120
Status	Completed
Type	Research Grant
Start date	18/09/2003
End date	30/09/2004
Duration	13 months

Abstract

Numerous biochemical studies, both pre- and post-genomic, rely on the ability to discriminate moietiest that are bound to macromolecules or to small molecule from molecules of the same type but which are free in solution. This applies particularly to protein-protein interactions, to protein-nucleic acid interactions and to protein-ligand interactions, but is also true e.g. when considering the incorporation of low-MW precursors into macromolecules. Preferred methods for making such measurements are homogeneous (i.e. do not require a separation step) and can be done in tiny volumes so as to conserve precious home-isolated or expensive commercial reagents. The ideal method would measure single molecules. Methods based on Fluorescence Correlation Spectroscopy (FCS) and Fluorescent Intensity Distribution Analysis (FIDA) fall into this category and BBSRC have funded an instrument in the PIs lab. We now wish to extend the capability of this instrument, and to make its use more easily and widely available to colleagues, as follows: (a) to allow nanolitre dispensing of reagents into special nanowell plates designed for use with the instrument, (b) to add a fluorescence lifetime analysis (cFLA) capability to instrument to discriminate different mechanisms by which the fluorescence yield can change, and (c) to acquire the software necessary for doing the new Fluorescence Intensity Lifetime Distribution Analysis (FILDA). The equipment will be applied to a host of BBSRC-funded projects involving such low-volume, high-sensitivity measurements in Manchester.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2003 (RE2) [2003]
Funding Scheme	X – not Funded via a specific Funding Scheme

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