BBSRC Portfolio Analyser
Award details
Monitoring environmental oxygen: FNR a sensor of oxygen and oxidative stress
Reference
PRS12148
Principal Investigator / Supervisor
Professor J Green
Co-Investigators /
Co-Supervisors
Professor Andrew Thomson
Institution
University of Sheffield
Department
Molecular Biology and Biotechnology
Funding type
Research
Value (£)
191,538
Status
Completed
Type
Research Grant
Start date
01/01/2000
End date
01/01/2003
Duration
36 months
Abstract
Monitoring environmental oxygen concentrations is of fundamental importance to bacteria. FNR, the major oxygen-responsive transcription factor of E. coli, senses oxygen directly via the assembly-disassembly of an oxygen-labile [4Fe-4S) cluster. We aim to investigate (1) the molecular basis of the FNR switch and its physiological consequences, using a range of spectroscopic probes to monitor the response of the Fe-S clusters of FNR to oxygen, combined with assays (gel retardation, footprinting and in vitro transcription) of biological function; (2) the signal transduction pathway using FNR-linked probes (EPR and fluorescence) of protein conformation; and (3) the role of FNR as an aerobic regulator by 2D PAGE and DNA/mRNA binding assays.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Prokaryotic Responses to Environmental Stress (PRS) [1999]
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search