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Characterisation of the novel cell wall remodelling required for vancomycin resistance in Streptomyces

Reference	P20040
Principal Investigator / Supervisor	Professor Mark Buttner
Co-Investigators / Co-Supervisors	Dr Hee-Jeon Hong
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	194,845
Status	Completed
Type	Research Grant
Start date	01/09/2003
End date	31/08/2006
Duration	36 months

Abstract

We have discovered a cluster of seven from S. coelicolor that confers inducible, high-levl vancomycin resistance, and genetic analysis suggests that this resistance is achieved by remodelling the cell wall peptidoglycan in a novel way. In particular, we have shown that a novel resistance gene (vanF), predicted to encode an enzyme involved in remodelling the peptide crossbridge, is required for vancomycin resistance. The objectives of this application are: (i) to determing the structure of S. coelicolor peptidoglycan; (ii) to characterise the peptidoglycan remodelling that occurs in response to vancomycin; (iii) to define the biochemical role of VanF; and (iv) to identify the specific ligand that is recognised by the VanS senor kinase.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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