Award details

Sensing and responding to oxidative stress through RsrA-sigma R in streptomyces

Reference	P11071
Principal Investigator / Supervisor	Professor Mark Buttner
Co-Investigators / Co-Supervisors	Dr Mark Paget
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	179,020
Status	Completed
Type	Research Grant
Start date	01/03/1999
End date	30/04/2003
Duration	50 months

Abstract

We have identified two proteins sigma R and RsrA (an anti-sigma factor), that modulate expression of the thioredoxin system in response to oxidative stress in S. coelicolor. The objectives of the proposed work are: (i) to identify by site-directed mutagenesis which of the 7 Cys residues are involved in the formation of the intramolecular disulphide bond that inactivates RsrA; (ii) to confirm this assignment through MALDI-TOF spectroscopic analysis of tryptic peptides generated from the reduced and oxidised form of RsrA (from which the 5 other Cys residues have been removed); (iii) to confirm the in vitro biochemical data by examining the activity of the rsrA mutant alleles in vivo; (iv) to identify further genes under sigma R control; (v) to determine if oxidised RsrA is a substrate for the thioredoxin system; (vi) to examine the effect of up-regulating tr BA expression on the yield of the ACV-derived Beta-lactam cephamycin C in S. clavuligerus.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search