Award details

Examination of resistance to hyphal breakage in actinomycetes

Reference	P07291
Principal Investigator / Supervisor	Professor Michael Bushell
Co-Investigators / Co-Supervisors
Institution	University of Surrey
Department	Health and Medical Sciences
Funding type	Research
Value (£)	205,919
Status	Completed
Type	Research Grant
Start date	11/01/1997
End date	11/01/2000
Duration	36 months

Abstract

We have established that hyphal particle size distribution affects secondary metabolite production in Actinomycete cultures. There appears to be a minimum particle size for production and high fragmentation rates lead to decreased production despite growth being unaffected. Disposition to fragmentation is determined by peptidoglycan synthesising enzyme activity. We propose to study this latter observation, using mutants, to assess the importance of each stage of peptidoglycan biosynthesis to hyphal strength and consequent product yield, thereby identifying generic targets for rational strain improvement. The influence of branching rate on breakage rate, importance of inoculum hyphal size distribution on batch:batch variability and real-time morphology manipulation for enhanced product yield will also be studied. A complementary programme of culture image analysis and hyphal tensile strength measurement will be undertaken at Birmingham University. (Joint with grant P07303).

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

P07303 Examination of resistance to hyphal breakage in actinomycetes

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