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The role of an alternative sigma factor of Xanthomonas campestris in lipopolysaccharide biosynthesis and pathogenicity

Reference	P05167
Principal Investigator / Supervisor	Professor Michael Daniels
Co-Investigators / Co-Supervisors	Professor Mark Buttner, Dr Max Dow
Institution	University of East Anglia
Department	Sainsbury Laboratory
Funding type	Research
Value (£)	121,630
Status	Completed
Type	Research Grant
Start date	16/02/1996
End date	16/12/1999
Duration	46 months

Abstract

The aims of this work are to provide direct evidence that the product of a previously characterised pathogenicity gene rfaY is an RNA polymerase sigma factor, to identify genes whose transcription this factor controls and to test their role in host-pathogen interactions. This work will involve over-expression of rfaY, purification of the gene product and its use in in vitro transcription assays. Genes whose transcription depends upon RfaY will be mutagenised with Tn5 or Tn5lac and effects on pathogenicity and lipopolysaccharide biosynthesis assessed.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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