Award details

Elucidation of the anaerobic biosynthesis of vitamin B12

Reference	P04006
Principal Investigator / Supervisor	Professor Martin Warren
Co-Investigators / Co-Supervisors	Professor Peter Heathcote
Institution	University College London
Department	Institute of Ophthalmology
Funding type	Research
Value (£)	176,159
Status	Completed
Type	Research Grant
Start date	01/05/1995
End date	01/06/1998
Duration	37 months

Abstract

This project is aimed at investigating the anaerobic biosynthesis of vitamin B12 in Bacillus megaterium and Salmonella typhimurium. Using recombinant Escherichia coli strains harbouring expression plasmids containing the cobinamide biosynthetic genes from these organisms, a structural analysis of accumulated intermediates will be undertaken, thus elucidating the step-by-step conversion of precorrin-2 into cobinamide. This will clarify (i) the point at which cobalt enters the path, (ii) the method employed to cause ring contraction and (iii) the role of some of the proteins in these operons with no ascribed function.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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