Award details

Molecular insight into the structure and activity of plant enzymes involved in lipid biosynthesis

Reference	P02396
Principal Investigator / Supervisor	Professor Antony Slabas
Co-Investigators / Co-Supervisors	Dr P Engel, Professor David Rice
Institution	Durham University
Department	Biological and Biomedical Sciences
Funding type	Research
Value (£)	107,492
Status	Completed
Type	Research Grant
Start date	01/11/1994
End date	01/11/1997
Duration	36 months

Abstract

(1) To determine the 3-dimensional structure of enoyl reductase (ER) from rape using X-ray crystallography and to identify residues important in substrate binding and catalysis. (2) To carry out a detailed kinetic analysis of ER. (3) To identify important residues in ER through a programme of covalent modification and site directed mutagenesis. (4) To investigate the molecular basis of the reaction between ER and its acyl carrier protein substrate. (5) To produce large quantities of B-ketoreductase (B-KR) from rape in order to refine the conditions for crystallisation and for crystallisation trials and kinetic analysis. (Joint with grant P02370).

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

P02370 Molecular insight into the structure and activity of plant enzymes involved in lipid biosynthesis

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