Award details

Molecular insight into the structure and activity of plant enzymes involved in lipid biosynthesis

Reference	P02370
Principal Investigator / Supervisor	Professor David Rice
Co-Investigators / Co-Supervisors	Professor Peter Artymiuk, Professor Antony Slabas
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	157,161
Status	Completed
Type	Research Grant
Start date	01/02/1995
End date	01/02/1998
Duration	36 months

Abstract

1. To determine the 3-dimensional structure of enoyl reductase (ER) from rape using X-ray crystallography and to identify residues important in substrate binding and catalysis. 2. To carry out a detailed kinetic analysis of ER. 3. To identify important residues in ER through a programme of covalent modification and site-directed mutagenesis. 4. To investigate the molecular basis of the reaction between ER and its acyl carrier protein substrate. 5. To produce large quantities of B- ketoreductase (B-KR) from rape in order to refine the conditions for crystallisation and for kinetic analysis. (Joint with grant P02396).

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

P02396 Molecular insight into the structure and activity of plant enzymes involved in lipid biosynthesis

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