Award details

(A) Purchase of an abi prism 377 automated sequencer for genomic mapping of disease resistance genes

Reference	JRE10814
Principal Investigator / Supervisor	Dr N Bumstead
Co-Investigators / Co-Supervisors	Professor Jim Kaufman, Professor Venugopal Nair
Institution	The Pirbright Institute
Department	Div of Molecular Biology
Funding type	Research
Value (£)	48,000
Status	Completed
Type	Research Grant
Start date	01/03/1999
End date	01/09/1999
Duration	6 months

Abstract

In previous work we have developed tools and methodology which allow genomic mapping in chickens, with the long term aim of mapping genes involved in disease resistance. We have now used these tools to map novel genes for resistance to salmonellosis and to Mareks disease. These are the first genes to be mapped in this manner in chickens and are of considerable commercial and scientific importance. The successful mapping of these genes strongly suggests our genomic mapping approach can be applied to other disease resistance and immunological traits, and we have already identified genetic differences in these traits in preparation for this work. However our existing 373A automated sequencer is already in use continuously. The purchase of an ABI 377 sequencer will greatly expand our capacity for automated genotyping of microsatellite and AFLP loci and will allow us to analyse further disease resistance genes and also the larger numbers of animals necessary to investigate genetically more complex traits. Greater sequencing capacity will also allow us to analyse allelic differences in genes already identified, making it possible to develop efficient selection methods for variants in these genes and to greatly extend our present work relating the molecular effects of sequence differences to phenotype.

Summary

unavailable

Committee	Not funded via Committee
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Research Equipment Initiative 1998 (JE3) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search