Award details

(A) Purchase of a steady state fluorimeter for studies of protein folding

Reference	JRE10782
Principal Investigator / Supervisor	Professor Sheena Radford
Co-Investigators / Co-Supervisors
Institution	University of Leeds
Department	Inst of Molecular & Cellular Biology
Funding type	Research
Value (£)	16,808
Status	Completed
Type	Research Grant
Start date	01/04/1999
End date	01/10/1999
Duration	6 months

Abstract

Fluorescence spectroscopy is an essential technique for studies of protein folding. We will use fluorescence to measure (i) equilibrium denaturation transitions, (ii) the population and conformational properties of equilibrium partially folded states (measured by the binding of anilino naphthalene sulphonic acid (ANS), (iii) ligand binding to native molecules using fluorescently labelled substrates (peptides or RNA) and (iv) fluorescence anisotrophy of proteins at equilibrium in different conformational states. Four different projects will be make use of the newly purchased fluorimeter. These involve the understanding of protein folding mechanisms at a fundamental level; investigation of the substrate binding site in the molecular chaperone, GroEL; the unfolding of ricin and the role of partially folded states in toxicity; and the molecular mechanism of protein misfolding in amyloidosis. The topics embrace the field of protein folding in its widest perspective, linking studies of the fundamental principles of protein folding to the role of protein misfolding in disease. The purchase of a fluorimeter dedicated to equilibrium protein folding studies will underpin the results obtained from stopped flow fluorescence and will revolutionise our experimental productivity in all of these areas. In addition, it will allow vital new experiments involving fluorescence anisotrophy to begin.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Research Equipment Initiative 1998 (JE3) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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