Award details

(A) Enhanced mass spectrometry facility for pharmaceutical proteomics

Reference	JE514776
Principal Investigator / Supervisor	Dr Kevin Welham
Co-Investigators / Co-Supervisors	Dr Mike Munday, Professor Laurence Patterson, Professor Mark Searcey, Dr ANDREW WILDERSPIN
Institution	University College London
Department	Dept of Pharmaceutical and Biological Ch
Funding type	Research
Value (£)	35,676
Status	Completed
Type	Research Grant
Start date	27/04/2001
End date	27/06/2001
Duration	3 months

Abstract

This proposal is for equipment essential to our use of mass spectrometry for interrogation of whole cell or tissue protein expression (i.e. the proteome) in order to identify target protein structures and relate them to cellular activity. We have recently invested heavily in new MS equipment with the purchase of a ThermoQuest Finnigan LCQ Duo ion trap LC/MS/MS system. For proteomic studies it requires a nano- scale capillary LC system with autosampler coupled to a nanospray ion source in order for it to be efficiently used to provide detection at the 10-12 to the 10-15 molar levels required. The equipment requested is considered the best currently available and comprises an UltiMate nano-LC system, nano-spray ion source and additional software updates to provide a modern, flexible and automated approach for the sequencing and searching of data. The equipment requested will provide an integrated system with high levels of automation, all controlled through the existing mass spectrometers data system. It will be available to a multi-user group particularly interested in the influence of pharmaceuticals on protein expression namely (i) Mass spectrometric studies on biopolymer structure and function; (ii) Proteomics of tumour cytochrome P450 targeted novel chemotherapy; (iii) Protein Expression and Phosphorylation in the Mechanism of Action of Lipid-Lowering Drugs; and (iv) Protein-structure-based Drug Design.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 2000 (JE5) [2000]
Funding Scheme	X – not Funded via a specific Funding Scheme

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