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A concerted approach to global mutational analysis of gene function in Streptomyces coelicolor

ReferenceIGF12436
Principal Investigator / Supervisor Professor Margaret Smith
Co-Investigators /
Co-Supervisors		 Professor Keith Chater, Professor Paul Dyson
Institution University of Nottingham
DepartmentSchool of Molecular Medical Sciences
Funding typeResearch
Value (£) 235,868
StatusCompleted
TypeResearch Grant
Start date 01/07/2000
End date 01/07/2003
Duration36 months

Abstract

We will provide resources for systematic analysis of gene function in S. coelicolor. Transposon libraries containing random insertions will be phenotypically characterised and the disrupted genes identified. PCR based procedures will be developed for large- scale targeted mutagenesis by gene replacement with linear cassettes. Phage based vectors for insertional mutagenesis will be developed and exploited to insert regulated promoters to control essential genes, and to transduce insertions to form multiple mutants. Detailed functional analysis will focus on putative regulatory genes. All resources and methods will be made widely available, together with access to a co- ordinated database of all mutants, linked to existing S. coelicolor databases. (Joint with grants 58/IGF12431 and 208/IGF12432.)

Summary

unavailable
Committee Closed Committee - Genes & Developmental Biology (GDB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative Investigating Gene Function (IGF) [1999-2005]
Funding SchemeX – not Funded via a specific Funding Scheme
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