BBSRC Portfolio Analyser

Award details

A concerted approach to global analysis of gene function in Streptomyces coelicolor

ReferenceIGF12432
Principal Investigator / Supervisor Professor Keith Chater
Co-Investigators /
Co-Supervisors		 Professor Paul Dyson, Professor Margaret Smith
Institution John Innes Centre
DepartmentMolecular Microbiology
Funding typeResearch
Value (£) 390,316
StatusCompleted
TypeResearch Grant
Start date 03/04/2000
End date 03/04/2003
Duration36 months

Abstract

We will provide resources for systematic analysis of gene function in S. coelicolor. Transposon libraries containing random insertions will be phenotypically characterised and the disrupted genes identified. Linear cassettes generated by PCR will be used for targeted gene replacement. Phage based vectors for insertional mutagenesis will be developed and exploited to insert regulated promoters to control essential genes, and to transduce insertions to form multiple mutants. Detailed functional analysis will focus on putative regulatory genes. All resources and methods will be made widely available, together with access to a co-ordinated database of all mutants, linked to existing S. coelicolor databases. (Joint with grants 58/IGF12431 and 42/IGF12436.)

Summary

unavailable
Committee Closed Committee - Genes & Developmental Biology (GDB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative Investigating Gene Function (IGF) [1999-2005]
Funding SchemeX – not Funded via a specific Funding Scheme
terms and conditions of use (opens in new window)
export PDF file
back to list new search