Award details

Role of DNA methylation as a determinant of successful epigenetic reprogramming by nuclear transfer

Reference	GTH14115
Principal Investigator / Supervisor	Professor Richard Meehan
Co-Investigators / Co-Supervisors	Professor Adrian Bird, Professor Ian Wilmut, Professor Lorraine Young
Institution	University of Edinburgh
Department	Inst of Cell Biology
Funding type	Research
Value (£)	186,448
Status	Completed
Type	Research Grant
Start date	01/11/2000
End date	31/03/2004
Duration	41 months

Abstract

Nuclear transfer using differentiated mammalian cells requires reprogramming of all lineage-specific epigenetic modifications for viable offspring to result. The likely role of DNA methylation in the reprogramming process will be investigated in the sheep, where changes in DNA methylation during embryogenesis have not been studied. An understanding of the regulation and possible deregulation of DNA methylation in this species will have important implications for improving the efficiency and the normality of nuclear transfer for both indirect (through animal products) and direct (through human cell-based therapies) healthcare applications. We will examine methylation in donor cells, oocyte cytoplasm, normal and reconstructed embryos. (Joint with grant 215/GTH14114.)

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Gene Technologies Underpinning Healthcare (GTH) [1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

GTH14114 Role of DNA methylation as a determinant of successful epigenetic reprogramming by nuclear transfer

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