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Genomic and proteomic analysis of PI3K-targets

Reference	GAN13064
Principal Investigator / Supervisor	Dr Phillip Hawkins
Co-Investigators / Co-Supervisors	Professor Len Stephens
Institution	Babraham Institute
Department	Babraham Institute Department
Funding type	Research
Value (£)	284,904
Status	Completed
Type	Research Grant
Start date	01/04/2000
End date	31/07/2003
Duration	40 months

Abstract

The 3-phosphorylated phosphoinositides (3-PPIs) products of phosphoinositide 3- kinases (P13Ks) are thought to be a family of intracellular signalling molecules. We have developed affinity supports carrying the four 3-PPIs and used them to isolate proteins we predict are possible P13K-effectors. So far we have used two supports and characterised 35 proteins from various mammalian cells. By sequencing and fast-action-bombardment-MS we have identified 20 proteins; 6 define new gene families, 14 being either new members of known families or known proteins. We plan to: 1) more completely exploit these reagents to identify more proteins; 2) use the accumulated sequence and binding data to define generic lipid binding consensus' with which to further trawl databases; and 3) use a combination of targeted-disruption, antisense and dominant-negative constructs and specific inhibitory antibodies to define the function of the genes we identify.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Genomics in Animal Function Initiative (GAN) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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