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Probing histone acetyl transferase function by directed evolution
Reference
G20017
Principal Investigator / Supervisor
Professor Florian Hollfelder
Co-Investigators /
Co-Supervisors
Dr Karl Nightingale
Institution
University of Cambridge
Department
Biochemistry
Funding type
Research
Value (£)
201,956
Status
Completed
Type
Research Grant
Start date
01/04/2004
End date
31/08/2007
Duration
41 months
Abstract
The modification of chromatin structure (e.g. by acetylation) of the histone `tails¿ plays a central role in transcriptional regulation. Currently the dissection of the roles of individual modified histone lysine residues is limited by low activity and/or specificities of isolated/recombinant histone acetyl transferases (HATs). We propose to combine directed evolution with biochemical and in vivo assays to understand the functional consequences of modifying specific lysine residues. Initially, we will use natural HATs (EsaI and MOF) as starting points to evolve novel HATs that have enhanced activities and altered specificities to probe biological function.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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