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DNA topoisomerase VI anarchael enzyme with a role in cell-size controlcell cycle and endoreduplication in Arabidopsis

Reference	G18653
Principal Investigator / Supervisor	Professor Anthony Maxwell
Co-Investigators / Co-Supervisors	Professor Keith Roberts, Dr Keiko Sugimoto-Shirasu
Institution	John Innes Centre
Department	Biological Chemistry
Funding type	Research
Value (£)	222,384
Status	Completed
Type	Research Grant
Start date	15/01/2003
End date	14/07/2006
Duration	42 months

Abstract

Two extreme dwarf mutants of Arabidopsis, root hairless2 (rhl2) and hypocotyl6 (hyp6), are deficient in endoreduplication, a process that occurs in most eukaryotes and involves genome amplification without cell division. We have recently demonstrated, by map-based cloning, that RHL2 and HYP6 encode subunits, A and B, respectively, of the putative DNA topoisomerase VI (topo VI). We have obtained preliminary evidence that topo VI has a role in cell-size control, cell cycle and endoreplication. To understand the role of this archaea- and plant- specific DNA topoisomerase in these processes, we will characterise its biochemical properties in vitro, and study the expression patterns and sub-cellular localisation/dynamics of RHL2 and HYP6. We will identify proteins that interact with topo VI by genetic, molecular biological and biochemical approaches.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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