Award details

Functional analysis of highly conserved unidentified reading frames in the E. coli genome

Reference	G12163
Principal Investigator / Supervisor	Professor Douglas Kell
Co-Investigators / Co-Supervisors
Institution	Aberystwyth University
Department	Inst of Biological, Environ & Rural Sci
Funding type	Research
Value (£)	191,408
Status	Completed
Type	Research Grant
Start date	01/08/1999
End date	01/08/2002
Duration	36 months

Abstract

Our computer analysis indicates that E. coli possesses 63 genes that are both highly conserved in evolution and functionally undefined. These 63 conserved- unidentified reading frames (cURFs) are likely to have important functions in bacteria and, in some cases, eukaryotes also. We propose to investigate the roles that these 63 cURFs play in E. coli. The proposed project involves (i) inactivation of target genes; (ii) investigation of the phenotypes of resulting mutants by assessing their growth and stress resistance; (iii) assessment of the effects that the mutations have on protein (proteome) and small-molecule (metabolome) compositions. The work described will give information on 63 novel conserved genes. Findings will be relevant to other bacteria and in many cases to eukaryotic systems. By focusing on these most highly conserved URFs, the approach described represents an efficient way to exploit the genome sequence data generated for E. coli and other micro- organisms to make new discoveries concerning gene function that will highlight new antimicrobial targets. (Joint with award G11549.)

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

G11549 Functional analysis of highly conserved unidentified reading frames in the E. coli genome

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