Award details

Functional analysis of highly conserved unidentified reading frames in the E. coli genome

ReferenceG11549
Principal Investigator / Supervisor Professor Simon C Andrews
Co-Investigators /
Co-Supervisors
Institution University of Reading
DepartmentAnimal and Microbial Sciences
Funding typeResearch
Value (£) 203,725
StatusCompleted
TypeResearch Grant
Start date 01/08/1999
End date 01/11/2002
Duration39 months

Abstract

Our computer analysis indicates that E. coli possesses 63 genes that are both highly conserved in evolution and functionally undefined. These 63 conserved- unidentified reading frames (cURFs) are likely to have important functions in bacteria and, in some cases, eukaryotes also. We propose to investigate the roles that these 63 cURFs play in E. coli. The proposed project involves (i) inactivation of target genes; (ii) investigation of the phenotypes of resulting mutants by assessing their growth and stress resistance; (iii) assessment of the effects that the mutations have on protein (proteome) and small-molecule (metabolome) compositions. The work described will give information on 63 novel conserved genes. Findings will be relevant to other bacteria and in many cases to eukaryotic systems. By focusing on these most highly conserved URFs, the approach described represents an efficient way to exploit the genome sequence data generated for E. coli and other micro- organisms to make new discoveries concerning gene function that will highlight new antimicrobial targets. (Joint with award G12163.)

Summary

unavailable
Committee Closed Committee - Genes & Developmental Biology (GDB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
terms and conditions of use (opens in new window)
export PDF file