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Industrial biocatalysts by design: rational re-engineering of flavocytochrome p450 BM3 for chiral chemical synthesis

Reference	E20186
Principal Investigator / Supervisor	Professor Gordon Roberts
Co-Investigators / Co-Supervisors	Professor Paul Cullis, Professor Andrew Munro, Dr Andrew Westlake
Institution	University of Leicester
Department	Biochemistry
Funding type	Research
Value (£)	243,929
Status	Completed
Type	Research Grant
Start date	01/01/2004
End date	31/12/2006
Duration	36 months

Abstract

We will use rational and localised random mutagenesis of the mono-oxygenase P450 BM3 to generate catalysts that hydroxylate/epoxidate key molecules, providing chiral intermediates for complex organic synthesis. Our previous work shows the feasibility of re-designing BM3 to alter its substrate specificity and the regio- and stereoselectivity of oxidation. Molecular biology, enzymology and structural biology will be combined to engineer biotechnologically-exploitable variants. Specific targets to be addressed include 6,8-dihydoxyoctanoate (lipoate synthesis); indan-1,2-diol (protease inhibitors), 3,4-epoxybutanoate (carnitine synthesis) and styrene epoxides for synthesis of, e.g., beta-adrenergic agonists.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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