Award details

A general method to isolate multiple protein assemblies

Reference	E18957/2
Principal Investigator / Supervisor	Dr James Dowden
Co-Investigators / Co-Supervisors	Professor Melanie Welham
Institution	University of Nottingham
Department	Sch of Chemistry
Funding type	Research
Value (£)	120,813
Status	Completed
Type	Research Grant
Start date	04/07/2005
End date	03/12/2006
Duration	17 months

Abstract

Developing generic reagents for high throughput studies of protein- protein interactions is the main goal of this research. Though attractive, cross-linking of protein complexes requires extensive optimisation as well as prior knowledge of at least one member of a multiple protein assembly. Molecular design and chemical synthesis will be used to create molecules that overcome these barriers. Prototypes will be tested against known protein complexes in vitro, assessed by gel electrophoresis and mass spectrometry and improved. Application of these agents to cells followed by affinity purification and general global analysis is the ultimate objective.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

E18957 A general method to isolate multiple protein assemblies

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