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Mechamisms underlying stability/instability of industrially-relevant recombinant mammalian cells

Reference	E18788
Principal Investigator / Supervisor	Professor Alan Dickson
Co-Investigators / Co-Supervisors	Professor Stephen Oliver
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	274,548
Status	Completed
Type	Research Grant
Start date	01/09/2003
End date	31/08/2006
Duration	36 months

Abstract

The GS-NS0 system is an industrially-relevant stable expression system, especially for production of humanised recombinant antibodies. 30-50 per cent of all GS-NS0 clones exhibit rapid loss of recombinant protein expression, corresponding to loss of mRNA expression. This occurs despite maintenance of recombinant gene copy number and integrity. We will identify the stage at which changes to mRNA expression is determined (eg transcriptional or post-transcriptional) and define (via microarray and proteomics analyses) phenotypic characteristics that determine the propensity for clone-specific expression of stability or instability. The information obtained will have significant immediate value, offering the possibilities of predictive indicators of stable/unstable clones, and, in a longer timeframe, for engineering reproducible and efficient cell line production.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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