Award details

Systematic assignment of function to human genes by complementation in yeast.

ReferenceE18397
Principal Investigator / Supervisor Dr James Ross Miller
Co-Investigators /
Co-Supervisors
Institution Babraham Institute
DepartmentLab of Functional Immunogenteics
Funding typeResearch
Value (£) 191,340
StatusCompleted
TypeResearch Grant
Start date 01/04/2002
End date 31/03/2005
Duration36 months

Abstract

More than 40 per cent of all human proteins do not have an assigned function, no matter how tenuous. Previously, we set up a system to assign function to human genes by deriving a panel of yeast strains in which different essential genes had undergone promoter-substitution. We then used entire human cDNA libraries to rescue the down-regulated yeast strain by functional complementation. The new project will build on the knowledge gained in our initial project. We will increase the number of human cDNAs by including recently developed full-length collections and additional libraries. This should allow us to include splice variants. The screening procedure used will be streamlined, with resulting increase in throughput. Where possible we will analyse multiple complementing clones as these can define the minimum region of a protein responsible for functional complementation.

Summary

unavailable
Committee Closed Committee - Engineering & Biological Systems (EBS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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