Award details

Systematic assignment of function to human genes by complementation in yeast

Reference	E17601
Principal Investigator / Supervisor	Professor Stephen Oliver
Co-Investigators / Co-Supervisors	Dr James Ross Miller, Dr Michael Osborn
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	184,956
Status	Completed
Type	Research Grant
Start date	01/01/2003
End date	01/01/2006
Duration	36 months

Abstract

More than 40 per cent of all human proteins do not have an assigned function, no matter how tenuous. Previously we set up a system to assign function to human genes by deriving a panel of yeast strains in which different essential genes had undergone promoter-substitution. We then used entire human cDNA libraries to rescue the down-regulated yeast strain by functional complementation. The new project will build on the knowledge gained in our initial project. We will increase the number of human cDNAs by including recently developed full-length collections and additional libraries. This should allow us to include splice variants. The screening procedure used will be streamlined, with resulting increase in throughput. Where possible we will analyse multiple complementing clones as these can define the minimum region of a protein responsible for functional complementation.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

E18397 Systematic assignment of function to human genes by complementation in yeast.

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