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Interrogating DNA duplex-ligand interactions: a dynamic approach exploiting the intrinsic conductivity of duplex DNA
Reference
E16574
Principal Investigator / Supervisor
Professor Julea Butt
Co-Investigators /
Co-Supervisors
Dr Richard Bowater
,
Professor Andrew Mayes
Institution
University of East Anglia
Department
Chemistry
Funding type
Research
Value (£)
170,632
Status
Completed
Type
Research Grant
Start date
12/08/2002
End date
11/02/2005
Duration
30 months
Abstract
The sensitivity of duplex DNA conductivity to perturbation of pi-stack integrity provides a powerful opportunity to probe DNA-ligand interactions. We propose to measure rates of long-range charge transport in DNA duplexes on gold electrodes by applying dynamic electrochemical methods. In-situ SPR analysis will provide independent quantitation of duplex-ligand binding; a reference for the electrochemical approach. Various duplex structures and duplex-ligand complexes will be interrogated to correlate rates of duplex-supported charge transport with defined structural perturbations. The knowledge will be applied to understanding supported charge transport with defined structural perturbations. The knowledge will be applied to understanding interactions of new DNA-binding molecules, in particular a real-time study of duplex recognition by DNA ligase.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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