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Strategic engineering of morphinone reductase and PETN reductase for biotransformation and biosensor applications

Reference	E09216
Principal Investigator / Supervisor	Professor Nigel Scrutton
Co-Investigators / Co-Supervisors	Professor Neil Bruce, Professor Peter Moody
Institution	University of Leicester
Department	Biochemistry
Funding type	Research
Value (£)	173,786
Status	Completed
Type	Research Grant
Start date	01/08/1998
End date	01/08/2001
Duration	36 months

Abstract

This project is aimed at investigating the structure and function of two closely related enzymes, morphinone reductase and PETN reductase, and engineering both enzymes to improve their applications in biotransformations and sensors. The physical chemical parameters of the enzymes which require changing are the cofactor specificity of morphinone reductase, and the substrate specificity of PETN reductase. We propose to obtain 3D structural data for the two enzymes at atomic resolution in order to pursue rational protein engineering to improve these systems. Work will also be directed towards randomly mutating libraries containing the PETN reductase gene (onr) from which variants will be selected that possess improved specificity. (Joint with grant 91/E09215) (MOD Joint-grant scheme)

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

E09215 Strategic engineering of morphinone reductase and PETN reductase for biotransformation and biosensor applications

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