Award details

Anticarcinogenic activity of crucifers: transcriptome analysis of human colon cells following exposure to extracts of broccoli and Arabidopsis

ReferenceD20475
Principal Investigator / Supervisor Professor David Barrett
Co-Investigators /
Co-Supervisors
Dr Jeff Fry
Institution University of Nottingham
DepartmentSch of Pharmacy
Funding typeResearch
Value (£) 49,744
StatusCompleted
TypeResearch Grant
Start date 01/04/2003
End date 31/03/2006
Duration36 months

Abstract

Epidemiological and physiological studies have provided substantial evidence that consumption of crucifers significantly reduces the risk of cancer. Several possible mechanisms have been proposed, many of which implicate glucosinolates and their degradation products, which include isothiocyanates (ITCs) and indolyl compounds. We propose to study changes in gene expression in human colon cell cultures with Affymetrix Human GeneChipTM U133 microarrays, following treatment with extracts of broccoli and isothiocyanate-enriched broccoli, and pure compounds. We will also compare the effect of broccoli extracts on gene expression with that of Arabidopsis extracts, and evaluate the potential to develop Arabidopsis as a model system to study the anticarcinogenic effect of crucifers due to its amenability to genetic modification. (Joint with grant 18003)

Summary

unavailable
Committee Closed Committee - Agri-food (AF)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
terms and conditions of use (opens in new window)
export PDF file