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Anticarcinogenic activity of crucifers: transcriptome analysis of human colon cells following exposure to extracts of broccoli and Arabidopsis

Reference	D18003
Principal Investigator / Supervisor	Professor Richard Mithen
Co-Investigators / Co-Supervisors	Dr John Rossiter
Institution	Quadram Institute Bioscience
Department	Nutrition Division
Funding type	Research
Value (£)	200,752
Status	Completed
Type	Research Grant
Start date	18/07/2003
End date	17/07/2006
Duration	36 months

Abstract

Epidemiological and physiological studies have provided substantial evidence that consumption of crucifers significantly reduces the risk of cancer. Several possible mechanisms have been proposed, many of which implicate glucosinolates and their degradation products, which include isothiocyanates (ITCs) and indolyl compounds. We propose to study changes in gene expression in human colon cell cultures with Affymetrix Human GeneChipTM U133 microarrays, following treatment with extracts of broccoli and isothiocyanate-enriched broccoli, and pure compounds. We will also compare the effect of broccoli extracts on gene expression with that of Arabidopsis extracts, and evaluate the potential to develop Arabidopsis as a model system to study the anticarcinogenic effect of crucifers due to its amenability to genetic modification.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

D20475 Anticarcinogenic activity of crucifers: transcriptome analysis of human colon cells following exposure to extracts of broccoli and Arabidopsis

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