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Single nucleotide polymorphisms in the promoter of a candidate gene for beef tenderness and effects on expression

Reference	D20453
Principal Investigator / Supervisor	Professor Tim Parr
Co-Investigators / Co-Supervisors	Dr Ronald Bardsley, Professor Peter Buttery, Dr Paul Sensky
Institution	University of Nottingham
Department	Sch of Biosciences
Funding type	Research
Value (£)	283,095
Status	Completed
Type	Research Grant
Start date	01/02/2004
End date	31/01/2007
Duration	36 months

Abstract

Biochemical and genetic studies indicate that activity of muscle calpastatin 24h after slaughter correlates inversely with beef tenderness, but factors that regulate calpastatin gene expression are unknown. We recently identified 3 porcine calpastatin promoters associated with exons 1xa, 1xb, 1u (as in mouse). The published bovine promoter is incomplete, lacking the 1u sequence which in pigs is used predominantly in skeletal muscle. We propose to isolate the bovine 1u promoter, test its functionality in vitro and screen for Single Nucleotide Polymorphisms (SNPs). We will test the hypothesis that SNPs present will affect the expression of the gene and hence beef quality. DNA and RNA will be obtained from animals of known meat quality in collaboration with the Roslin Institute.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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