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In situ detection of mutagenic compounds in soil using DNA adduct analysis

Reference	D18214
Principal Investigator / Supervisor	Professor Ian Singleton
Co-Investigators / Co-Supervisors	Professor Ken Killham
Institution	Newcastle University
Department	Sch of Biology
Funding type	Research
Value (£)	134,136
Status	Completed
Type	Research Grant
Start date	02/06/2003
End date	02/06/2005
Duration	24 months

Abstract

There is a need to assess the bioavailability of mutagenic compounds in soils as an aid to risk assessment and bioremediation. Current bioavailability methods use soil extracts which do not accurately reflect compound bioavailability. We propose to use soil DNA to assess the bioavailability of polycyclic aromatic hydrocarbons by measuring the amount of PAH-DNA adducts (mutagenic potential) on isolated DNA. The measurement of PAH-DNA adducts on isolated soil DNA is theoretically an in-situ measure of PAH bioavailablilty. Initial results have found DNA adducts in PAH contaminated soil and this research is designed to standardise the technique with a wide range of soils and compare DNA adduct results with other relevant bioavailability assays.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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