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The role of endotoxin (lipid A) in host-pathogen interactions in Salmonella infections of mice

ReferenceD09660
Principal Investigator / Supervisor Professor Duncan Maskell
Co-Investigators /
Co-Supervisors
Institution University of Cambridge
DepartmentVeterinary Medicine
Funding typeResearch
Value (£) 200,053
StatusCompleted
TypeResearch Grant
Start date 15/09/1998
End date 15/09/2001
Duration36 months

Abstract

We propose to study early molecular events leading to inflammation, focal lesion development and induction of protective immunity in Salmonella infections in mice. From very early, pre-immune stages of infection, through to host death or the induction of protective responses, bacterial components signal to host cells. We intend to concentrate on the role of lipid A in these events. To this end we have generated mutant salmonellae with defined alterations in lipid A structure and function. Using molecular biology and immunohistochemistry, we have shown that these mutants are profoundly altered in several important host-bacterial interactions, including the local induction within focal lesions of TNF alpha, IL-1 beta and inducible nitric oxide synthase. We plan to extend these studies to understand these events in molecular detail in vivo. This work will generate fundamental knowledge about the infectious disease process and may lead to novel intervention strategies. (Joint with grant D09661).

Summary

unavailable
Committee Closed Committee - Agri-food (AF)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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