Award details

Structural analysis of RAMPs and their role in CGRP binding

Reference	C20090
Principal Investigator / Supervisor	Dr David Poyner
Co-Investigators / Co-Supervisors	Professor Mark Wheatley
Institution	Aston University
Department	Sch of Life and Health Sciences
Funding type	Research
Value (£)	195,951
Status	Completed
Type	Research Grant
Start date	01/01/2004
End date	31/12/2006
Duration	36 months

Abstract

RAMPs form heterodimers with the G-protein-like receptor CRLR to yield receptors for CGRP and adrenomedullin. We will analyse the structure of RAMP1 by site directed mutagenesis and production of chimeric receptors with RAMP3 to identify key regions needed for glycosylation, structural integrity and the ability to interact with CRLR to produce a functional CGRP receptor. We will also investigate the conditions needed to produce a correctly-folded, soluble version of the extracellular domain of RAMP1 and characterise this with respect to both its structure (NMR, CD, FT-IR) and functions (interaction of CRLR to produce a CGRP receptor). (Joint with C20091)

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

C20091 Structural analysis of RAMPs and their role in CGRP binding

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