Award details

DNA gyrase from Arabidopsis thaliana

Reference	C17465
Principal Investigator / Supervisor	Professor Anthony Maxwell
Co-Investigators / Co-Supervisors	Dr Melisa Wall
Institution	John Innes Centre
Department	Biological Chemistry
Funding type	Research
Value (£)	212,396
Status	Completed
Type	Research Grant
Start date	01/06/2002
End date	31/05/2005
Duration	36 months

Abstract

We have obtained strong evidence for the existence of DNA gyrase in plastid and mitochondrial extracts of A. thaliana. This represents the first corroborated report of a gyrase in eukaryotes, and has important implications for drug targeting and organellar DNA replication in plants. We propose to clone, express and purify the enzyme and characterise its properties in vitro. We will determine its sensitivity to a range of topoisomerase inhibitors and investigate its role in organellar DNA replication. The aims are to understand the physiological role that this enzyme plays in plants, develop its potential as a herbicide target, and understand the evolutionary relationships between this enzyme and its bacterial counterparts.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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