Award details

Defining metal ion specificity and catalytic mechanism in tetrapyrrole chelatases

Reference	C15800
Principal Investigator / Supervisor	Professor Martin Warren
Co-Investigators / Co-Supervisors	Professor K Brocklehurst, Professor Peter Heathcote
Institution	Queen Mary University of London
Department	Sch of Biological and Chemical Sciences
Funding type	Research
Value (£)	213,360
Status	Completed
Type	Research Grant
Start date	01/01/2002
End date	31/07/2005
Duration	43 months

Abstract

The aims are to elucidate (i) the mechanism of metal ion chelation into tetrapyrroles and (ii) the process by which metal ion specificity is endowed by the chelatases. This will be achieved by studying the enzymes Met8p, a remarkable bifunctional dehydrogenase and chelatase associated with sirohaem synthesis, and CbiK, a chelatase associated with the anaerobic biosynthesis of cobalamin (vitamin B12). The mechanisms will be probed using a multidisciplinary approach involving site directed mutagenesis, regiospecific labelling of substrates, pH- dependent kinetic analysis, studies of metal binding and X-ray crystallography.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

C15800/2 Defining metal ion specificity and catalytic mechanism in tetrapyrrole chelatases

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