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Defining the repertoire of cell receptors utilised by human adenoviruses

Reference	C15265
Principal Investigator / Supervisor	Professor George Blair
Co-Investigators / Co-Supervisors	Dr Maria Blair
Institution	University of Leeds
Department	Inst of Molecular & Cellular Biology
Funding type	Research
Value (£)	202,868
Status	Completed
Type	Research Grant
Start date	01/03/2002
End date	01/03/2005
Duration	36 months

Abstract

Protein chemical and cDNA expression cloning approaches will be applied to identify, clone and characterise the cellular receptor(s) for Ad3. Chemical cross-linking studies and affinity chromatography experiments using recombinant Ad3 fibre knob and Ad3-permissive HeLa cells will be performed to determine the subunit molecular weight of receptor polypeptides. In a parallel approach, SV40-transformed Chinese Hamster Ovary (SV-CHO) cells, which do not bind Ad3 fibre or support viral infection, will be transiently transfected with a cDNA expression library derived from receptor-positive HeLa cells. Receptor- positive cells will be selected by binding fluorescently-labelled fibre followed by fluorescence activated cell sorting. Tissue distribution of the receptor will be studied by multiple-tissue Northern blotting and immunocytochemistry with antibodies raised either against recombinant receptor protein or chemically synthesised peptides.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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