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Structure/function analyses of engineered forms of flavocytochrome b2
Reference
C15007
Principal Investigator / Supervisor
Professor Stephen Chapman
Co-Investigators /
Co-Supervisors
Dr Daniel Craig
,
Professor Graeme Reid
,
Professor Malcolm Walkinshaw
Institution
University of Edinburgh
Department
Sch of Chemistry
Funding type
Research
Value (£)
185,384
Status
Completed
Type
Research Grant
Start date
01/10/2001
End date
26/01/2005
Duration
40 months
Abstract
Flavocytochrome b2 from S. cerevisiae is a lactate:cytochrome c oxidoreductase. We shall integrate protein engineering, crystallography, kinetics and potentiometric methods to provide a full structure/function analysis on two key areas of this enzyme system; substrate specificity and inter-domain recognition. The results from these studies will provide an unprecedented understanding of this system at a molecular level. Importantly, this work will provide a fundamental understanding of substrate selectivity in a whole family of related 2-hydroxyacid oxidising flavoenzymes. In addition, the project will make a significant contribution to our knowledge of how protein mobility affects intra-molecular electron transfer in proteins.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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