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Structure/function analyses of engineered forms of flavocytochrome b2

ReferenceC15007
Principal Investigator / Supervisor Professor Stephen Chapman
Co-Investigators /
Co-Supervisors
Dr Daniel Craig, Professor Graeme Reid, Professor Malcolm Walkinshaw
Institution University of Edinburgh
DepartmentSch of Chemistry
Funding typeResearch
Value (£) 185,384
StatusCompleted
TypeResearch Grant
Start date 01/10/2001
End date 26/01/2005
Duration40 months

Abstract

Flavocytochrome b2 from S. cerevisiae is a lactate:cytochrome c oxidoreductase. We shall integrate protein engineering, crystallography, kinetics and potentiometric methods to provide a full structure/function analysis on two key areas of this enzyme system; substrate specificity and inter-domain recognition. The results from these studies will provide an unprecedented understanding of this system at a molecular level. Importantly, this work will provide a fundamental understanding of substrate selectivity in a whole family of related 2-hydroxyacid oxidising flavoenzymes. In addition, the project will make a significant contribution to our knowledge of how protein mobility affects intra-molecular electron transfer in proteins.

Summary

unavailable
Committee Closed Committee - Biochemistry & Cell Biology (BCB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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