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Inhibition of cAMP-dependent protein kinase by the Hepatitis C virus NS3 protein: biochemical characterisation and consequences for hepatocyte physiology

Reference	C09593
Principal Investigator / Supervisor	Dr MPG Harris
Co-Investigators / Co-Supervisors	Dr Roger Clegg, Professor David Rowlands
Institution	University of Leeds
Department	Inst of Molecular & Cellular Biology
Funding type	Research
Value (£)	177,244
Status	Completed
Type	Research Grant
Start date	11/01/1999
End date	11/01/2002
Duration	36 months

Abstract

There is great interest in understanding the host/virus interactions that contribute to the pathogenesis and persistence of Hepatitis C Virus (HCV) with a view to generating novel anti-viral agents. The HCV non-structural protein NS3, as well as functioning as a protease and RNA helicase, can interact with and inhibit cAMP- dependent protein kinase (PKA). This observation has profound consequences for the physiology of infected hepatocytes as PKA plays a pivotal role in signal transduction pathways that control a number of key hepatocyte functions. This project aims firstly to express native NS3 in hepatocytes and assess the consequences of the interaction by analysing a number of key metabolic functions regulated by PKA. Secondly the physical interaction between the two proteins will be analysed in more detail using purified recombinant proteins.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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