Award details

Characterisation of the novel cell wall remodelling required for vancomycin resistance in Streptomyces

Reference	BBS/E/J/0000A165
Principal Investigator / Supervisor	Professor Mark Buttner
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	71,147
Status	Completed
Type	Institute Project
Start date	01/09/2003
End date	31/08/2006
Duration	36 months

Abstract

Vancomycin is the front-line therapy for treating problematic infections caused by MRSA, and the spread of vancomycin resistance is an acute problem. We have discovered a cluster of seven genes from S. coelicolor that confers inducible, high-level vancomycin resistance, and genetic analysis suggests that this resistance is achieved by remodelling the cell wall peptidoglycan in a novel way. In particular, we have shown that a novel resistance gene (vanF), predicted to encode an enzyme involved in remodelling the peptide crossbridge, is required for vancomycin resistance. The objectives of this grant application are (i) to determine the structure of S. coelicolor peptidoglycan (ii) to characterise the peptidoglycan remodelling that occurs in response to vancomycin (iii) to define the biochemical role of VanF (iv) to begin to explore why the remodelling specified by VanF is required for vancomycin resistance, and (v) to identify the specific ligand that is recognised by the VanS sensor kinase.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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